The biology of seeds: recent research advances: proceedings by G Nicolas, K J Bradford, D Come, H W Pritchard

By G Nicolas, K J Bradford, D Come, H W Pritchard

Complaints of the 7th overseas Workshop on Seed Biology held in Salamanca, Spain in could 2002. DLC: Seeds--Congresses.

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Benech-Arnold et al Fig. 3. Germination indices (GI) for embryos of IS 9530 (IS), Redland B2 (B2) and the hybrids that resulted from reciprocal crosses (IS × B2 and B2 × IS; symbols U and 0 indicate which genotype had acted as mother and as father, respectively, in the cross) harvested at different days after pollination (DAP) and incubated in presence of 5 µmol ABA at 25°C for 12 days. Data were not available for the cross B2 × IS on 32 DAP. Vertical bars show SE (n = 3). transcription factor that, among other processes, controls sensitivity to ABA.

Caryopses (40 DAP) were incubated in water for different lengths of time. (A) SbVp1specific northern hybridization to total RNA (25 µg) isolated from embryos of fluridone-treated and control caryopses (upper panel). The same filter was hybridized to an rRNA-specific probe (lower panel) to indicate RNA loading. (B) Germination percentages of 40 DAP caryopses from control (●, ■ ) and fluridone-treated panicles (●, ■ ) of IS 9530 (■, ■ ) and Redland B2 (●, ● ) varieties at different imbibition times.

Tissue preparation and microscopy were performed as described by Borisjuk et al. (1995, 2002a). Biochemical procedures as well as in situ techniques were performed according to Weber et al. (1997). Results The E2748 seed mutation affects embryo growth during the maturation phase Fresh weight of mutant and wild-type embryos is not different up to the stage when morphogenesis and cell division have terminated and maturation started. Mutant embryos grow slowly and reach only approximately 10% of the wild-type values (Fig.

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