By Sankar Adhya, Susan Garges
RNA polymerase is molecule very important to gene transcription. in addition to linked components, RNA polymerase is a part of the method during which RNA is transcribed to supply a protein. * building and purification of RNA polymerases * DNA microarrays and bacterial gene expression * useful research of transcription components
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Extra resources for RNA Polymerase and Associated Factors, Part C
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150 ml) of TGED þ 50 mM NaCl and is desalted rapidly by passage over a 600-ml P-6 desalting column (48 Â 4 cm; Bio-Rad) equilibrated previously in the same buffer. Note that Mg(II) is omitted to increase the stability of the DNA– cellulose during the subsequent chromatography step. Prior to running the DNA–cellulose affinity column, fractions from the initial stages of purification are analyzed by SDS–PAGE to determine if polymin P precipitation and elution led to a good recovery of RNAP. 4 M wash.
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