Redox Cell Biology and Genetics Part B by Chandan K. Sen, Lester Packer

By Chandan K. Sen, Lester Packer

The seriously acclaimed laboratory regular for greater than 40 years, Methods in Enzymology is without doubt one of the such a lot hugely revered guides within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless appropriate today—truly a necessary booklet for researchers in all fields of existence sciences.

  • Protein constitution and Function
  • Nucleic Acids and Genes

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Hydrated electrons react with N20. The kinetic scheme is the following: OH + HCOO- --+ H20 + COO'- (10) H + HCOO- ~ H2 + COO'- (11) eaq + N20 --+ OH + OH- + N2 (12) Thus all water free radicals are transformed into the reductant COO'- radical. 62 #mol J -1 In this expression g(H), g(OH), and g(e~q) denote the respective G values given in Table I. The whole process [reactions (10)-(12)] is finished in less than 1 #sec after interaction of the ionizing radiation. 19 j. W. T. Spinks and R. J. Woods, "Introduction to Radiation Chemistry," 3rd Ed.

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Rhee, J. Biol. Chem. 273, 15366 (1998). 6 S. Veeraraghava, C. C. Mello, E. J. Androphy,and J. D. Baleja, Biochemistry 38, 16115 (1999). 7 M. A. Gorman, S. Morera, D. G. Rothwell, E. de La Fortelle, C. D. Mol, J. A. Tainer, I. D. Hickson, and E S. Freemont, EMBO J. 16, 6548 (1997). 8 A. Claiborne, T. C. Mallett, J. I. Yeh,J. Luba, and D. Parsonage,Adv. Protein Chem. 58, 215 (2001). )"~ [5] 3 I--FAD L--SH (S') SCHEME 1 cystine per subunit and NADH reduction generates one Cys-SH. 9 In Npx, the cycling between the reduced thiol or thiolate and the oxidized sulfenate or sulfenic acid of residue 42 is an intrinsic part of the catalytic cycle (species 1 and 3, Scheme 1) as the formation of the catalytically competent thiolate form (species 2, Scheme 1) precedes the binding of H202.

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