Plant Protoplasts and Genetic Engineering III by Y. Asano (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

By Y. Asano (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

In continuation of Volumes eight and nine (1989) on in vitro manipulation of plant protoplasts, this new quantity offers with the regeneration of crops from protoplasts and genetic transformation in quite a few species of Agrostis, Arabidopsis, Atropa, Brassica, Catharanthus, Datura, Cucumis, Daucus, Digitalis, Duboisia, Eustoma, Festuca, Helianthus, Hordeum, Kalanchoe, Linum, Lobelia, Lolium, Lotus, Lycium, Lycopersicum,Mentha, Nicotiana, Pelargonium, Pisum, Pyrus, Salvia, Scopolia, and Solanum.These reviews mirror the some distance achieving implications of protoplast technologyin genetic engineering of vegetation. they're of specified curiosity to researchers within the box of plant tissue tradition, molecular biology, genetic engineering, and plant breeding.

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Plant protoplasts and genetic engineering IV. Springer, Berlin Heidelberg New York pp 170-182 Tsukada M, Kusano T, Kitagawa Y (1989) Introduction of foreign genes into tomato protoplasts by eiectroporation. Plant Cell Physiol 30:599-603 Ulrich TH, Chowdhury JB, Widholm JW (1980) Callus and root formation from mesophyll protoplasts of Brassica rapa. Plant Sci Lett 19:347-354 Wong KW, Loh CS (1987) In vitro regeneration of plantiets in Brassica alboglabra. Plant Cell Tissue Organ Cult 10: 143-148 Zee SY, Hui LH (1977) In vitro plant regeneration from hypocotyl and cotyledons of Chinese kale (Brassica alboglabra Bailey).

2-(N-morphino)ethanesulfonic acid, sodium salt. Eng-Chong Pua 24 3 Culture of Protoplasts The freshly isolated protoplasts (Fig. 2-1 x 106 jml PC medium (Table 2) (Pua 1987) in the dark for 5-7 days, then grown under dim light for the rest of the culture period prior to transfer to SI medium (Table 2) for shoot regeneration. Cell division generally begins 36-48 h after isolation (Fig. 2b). However, protoplast growth is markedly affected by the explant type and the growth regulator in the medium.

Belladonna (2n = 72) (Krumbiegel and Schieder 1979). Thirteen somatic hybrids were selected. Hybrid calli were recognizable by the production of hairs, typical of Datura, and the green color derived from Atropa. The chromosome number of hybrids varied from 84 to 175. In a subsequent study (Krumbiegel and Schieder 1981), somatic sexual incompatibility between D. innoxia and A. belladonna was compared. Experiments were carried out to test the feasibility of producing sexual hybrids through in vivo and in vitro methods by cross-pollination; however, no embryos, seeds or plantlets were obtained, thus demonstrating that protoplast fusion is the only possibility for obtaining hybrids between Datura and Atropa.

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