Osteoarthritis - Diagnosis, Treatment and Surgery by Q. Chen

By Q. Chen

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For accurate quantitative work, the thermal mass of the sample and reference pans were matched. The calorimetric properties of samples were determined by DSC method (Mettler-Toledo DSC 821e apparatus, Mettler-Toledo GmbH, Switzerland). Samples were heated from 0 to 80 °C. 3 °C/min. Conventional Hastelloy batch vessels were used with Characterization of Live and Experimentally Degenerated Hyaline Cartilage with Thermal Analysis 35 40 μl sample volume. All the DSC measurements were preceded in Ar atmosphere, and the flow rate was 100 ml/min.

4 Animal model Animal experiments were performed at the Physiology Department, Faculty of Medicine, University of Szeged. Adult male Wistar rats (Charles River strain; Bioplan, Budapest, Hungary), weighing 470±6 g were housed individually in ventilated cages on a 12-hour day/night cycle at a temperature of 22±1 °C. Water and food were provided ad libitum. The experiments were performed in accordance with the US National Institutes of Health Guidelines for the Care and Use of Laboratory Animals, and with the approval of the Animal Welfare Committee of the University of Szeged.

Fig. 1. g. enthalpy changes for reactions (ΔH) or phase changes. Calorimetry can also be used for qualitative and quantitative analyses. ΔH can often be determined for an unknown reaction in a complex system, and the value of ΔH can then be used to assist in identifying the reaction [Jones, 1997]. Since calorimetry directly measures the instantaneous rate of the process, calorimetry is a particularly advantageous method for determination of the kinetics of slow processes. Calorimetric parameters provide a description of the system as a function of the experimental variables.

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