: Normal Human Tissue and Cell Culture B . Endocrine, by Curtis C. Harris, Benjamin F. Trump and Gary D. Stoner

By Curtis C. Harris, Benjamin F. Trump and Gary D. Stoner (Eds.)

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Additional resources for : Normal Human Tissue and Cell Culture B . Endocrine, Urogenital, and Gastrointestinal Systems

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Transformations Occurred Monolayers; could be subcultured at least five times 69% or 333/484 explants produced squamous cells; 21/484: mixture of squamous cells contaminated with fibroblasts; 141484 were pure fibroblasts; 97 did not grow out (20%) Cultures held up well for 24 weeks, by morphologic criteria Measured colonial outgrowth and mitotic index. No cell line established Metaplastic epithelia covered all exposed surfaces within 12 weeks 38 GEORGE D. WILBANKS et al. 802OO), progesterone (Schering), testosterone (Matheson, Coleman & Bell TX 95), hydrocortisone (Sigma H-4001),and cortisone (Sigma C-2755).

29 29 30 31 31 38 40 47 47 49 50 I. Introduction A. Scope Most reports in the literature on the behavior of human uterine cervical tissues in vitro have focused mainly on abnormal material. For a review of cervical neoplasia in vitro, see Wilbanks and Fink (1976). These reports have generally lacked practical information on the tissue culture of normal cervical cells and have usually mentioned them briefly as controls for comparison with neoplastic cells. In addition, information on only a relatively small number of normal biopsies has been reported.

KAUFMAN et al. 1. HUMAN ENDOMETRIUM IN ORGAN CULTURE 21 organ culture for short intervals, usually less than 2 weeks. This time interval probably sufficed to enable the research goals of those studies to be accomplished. , 1969) may have discouraged further attempts at prolonged maintenance. We have observed a similar initial deterioration of the endometrial tissue in culture. However, since the goals of our studies appeared to require far longer periods of organ culture, we maintained the cultures beyond this interval of early deterioration.

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