By Milos Sovak
Read or Download Migrane-Therapie: Biofeedback, Akupunktur (Schriftenreihe Erfahrungsheilkunde) (German Edition) PDF
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Extra info for Migrane-Therapie: Biofeedback, Akupunktur (Schriftenreihe Erfahrungsheilkunde) (German Edition)
ICycler iQ Real-Time PCR Detection System (BioRad, Hercules, CA, USA) or equivalent. 4. Vector-specific primer set (see Note 4). 5. Vector Concentration and Gel Filtration Chromatography 1. , Bedford, MA, USA). 2. Superdex 200 10/300 GL gel filtration column (GE Healthcare). 3. ), 2 mM MgCl2 , 2% glycerol (see Note 5). 6. Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis The following sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) reagents are from Invitrogen (Carlsbad, CA, USA).
2. 96-well Blood DNA Purification Kits (Qiagen). 3. Cell Lysis 1. 96/384™ Multimek (Beckman Coulter, Fullerton, CA, USA). Other liquid handling robotic systems can be used for the lysis step. 2. 44 ml of 10% Triton X-100. This solution is prepared immediately before use. 3. Foil Seals are used to seal plates after lysis and before storage at –70 ºC. 4. PCR Quantitation 1. Primer Express Software (Applied Biosystems, Foster city, CA, USA) is used to design the primers and probes (see Note 1). 2.
And Sagar, S. L. (2005) Development of a purification process for adenovirus: controlling virus aggregation to improve the clearance of host cell DNA. Biotechnol. Prog. 21, 466–472. 10. , and Maheshwari, G. (2005) Inactivation of adenovirus type 5 by caustics. Biotechnol. Prog. 21, 446–450. 11. , and Maheshwari, G. (2004) Inactivation of adenovirus types 5 and 6 by Virkon S. Antiviral Res. 64, 27–33. Puriﬁcation of Adenovirus Vectors 23 12. , and Hsu, D. (2004) Thermal inactivation of adenovirus type 5.