Microbiology and Biochemistry of Strict Anaerobes Involved by Richard Sparling, Gerhard Gottschalk (auth.), Jean-Pierre

By Richard Sparling, Gerhard Gottschalk (auth.), Jean-Pierre Bélaich, Mireille Bruschi, Jean-Louis Garcia (eds.)

The trust that power will be a proscribing issue for the advance of humanity led 20 years in the past to a very good curiosity being'taken in learn on anaerobic digestion. the 1st foreign symposium held in Cardiff in 1979 used to be by means of the conferences in Travenmund (1981), Boston (1983), Guangzhou (1985) and Bologna (1988). by means of now anaerobic digestion has emerge as well-known as a suitable know-how for waste remedy. extra lately, the rise within the carbon dioxide content material of the ambience and (in built international locations, especially_ within the EEC) the· indisputable fact that an increasing number of land is turning into on hand for reasons except foodstuff creation make biomass creation economically and/or socially possible for commercial reasons. the opportunity of utilizing renewable natural carbon assets during this method is of serious strength curiosity for constructing organic recommendations and will significantly raise using anaerobic micro-organisms in cellulose biotransformation and effort construction from crop residues. This FEMS Symposium is dedicated to the interspecies hydrogen move phenomenon keen on the mineralization of natural subject in anaerobiosis. This strategy is conducted in Nature by means of consortia of anaerobic micro-organisms residing syntrophically. Many commercial purposes of those consortia as black containers for biogas construction and waste therapy were defined. even supposing those early ways have been fruitful, it sort of feels most likely higher wisdom on the molecular point of the extra attribute anaerobic micro organism which represent those consortia may drastically raise and enhance the usage of those organisms.

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Extra info for Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer

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Gigas, Hatchikian et al. (1978) describe it as a two subunit enzyme (M =-62000 and M = 26000) of molecular weight 89,500 with three Fe~S~ clu§ters. As showli in Table 1, these results provided an essentially complete and correct picture of the enzyme except for the now recognized essential presence of nickel. The amino acid composition of the D. gigas enzyme was found to be similar to that of the enzyme from D. , 1978), which is also known to be ~ subunit enzyme (M = 59000 and M = 28000; Yagi et al.

Eight of these are located in domain I and coordinate the two ferredoxin-clusters, while the remaining five in domain II could serve as ligands to the active site cluster. As discussed elsewhere (Voordouw, 1987), the hydA gene could have arisen by a gene fusion event. The discovery of hydC allows this hypothesis to be expanded and the discussion of the [Fe] hydrogenase genes in Desulfovibrio is concluded by considering the following path for their evolution: a) A gene encoding a hydrogen-binding polypeptide (domains II, III) fused with that encoding an 8Fe-8S ferredoxin to form the hydC-precursor gene.

Soc. , New Orleans (1989). 13. A. Kiener, C. Holliger, and T. Leisinger, Analogueresistant and auxotrophic mutants of Methanobacterium thermoautotrophicum. Arch. Microbiol. 139: 87-90 (1984). 14. A. Kiener, T. Rechsteiner, and T. Leisinger, Mutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl-tRNA synthetase. FEMS Microbiol. Lett. 33:15-18 (1986). 15. L. K. G. -P. Aubert, Isolation of auxotrophic mutants in support of ammonia assimilation via glutamine synthetase in Methanobacterium ivanovii.

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