By Jang B. Rampal
This moment half within the two-volume paintings Microarrays information purposes and information research. It contains perception into non-mammalian vertebrate platforms, procedures and protocols for top of the range glass-based microarrays. assurance contains purposes in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray structures in scientific perform, and screening of cDNA libraries on glass slide microarrays. Authors during this quantity additionally speak about protocols for predicting DNA duplex balance on oligonucleotide arrays and built-in research of microarray effects.
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Extra info for Microarrays: Volume 2, Applications and Data Analysis (Methods in Molecular Biology, v382)
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2005) Fabrication of chemical microarrays by efficient immobilization of hydrazide-linked substances on epoxide-coated glass surfaces. Angew. Chem. 117, 2941–2944. 19. This type of slide is commercially available. 20. Salisbury, C. , Maly, D. , and Ellman, J. A. (2002) Peptide microarrays for the determination of protease substrate specificity. J. Am. Chem. Soc. 124, 14,868–14,870. 21. , et al. (2004) Peptide protein microarrays for the simultaneous detection of pathogen infections. Bioconjugate Chem.
D) Dendrimercoated slides. The representation is simplified such that no structural effects are taken into account (molecular orientational effects, silane multilayer formation, cross-linking of polymers, and gel formation). Black spheres are linking groups introduced by the chemical synthesis of the slide. Gray spheres are reactive groups. On polymer slides of type 3C these groups might be either reactive functional groups or nonreactive groups, for example, amide. Structures are not drawn to scale.