Methods of Peptide Synthesis. The Peptides, Volume 1 by Eberhard Schröder and Klaus Lübke (Auth.)

By Eberhard Schröder and Klaus Lübke (Auth.)

Show description

Read or Download Methods of Peptide Synthesis. The Peptides, Volume 1 PDF

Best biochemistry books

Flavonoids. Chemistry, Biochemistry and Applications

Advances within the flavonoid box were not anything in need of staggering over the past two decades. whereas the clinical box has spotted flavonoids for his or her power antioxidant, anticancer and cardioprotectant features, growers and processors in plant sciences have applied flavonoid biosynthesis and the genetic manipulation of the flavonoid pathway in crops to enhance the dietary and decorative worth of plants.

Differential Display Methods and Protocols

Sporting at the excessive criteria of the 1st version of Differential demonstrate tools, Peng Liang et al. have established their moment version on a brand new mathematical version of differential exhibit (DD) that takes benefit of automation, in addition to electronic information acquisition and research. those well-versed authors clarify and spotlight the entire contemporary methodological refinements, together with automatic liquid dealing with of countless numbers of DD PCR response setups mixed with capillary electrophoresis, a prototype desktop software to immediately let confident band id from a fluorescence differential exhibit picture, and restrict fragment-based DD screenings which could hyperlink any cDNA fragment on to a given gene as soon as the series details of all transcripts turns into on hand.

The Next Generation in Membrane Protein Structure Determination

This ebook studies present options utilized in membrane protein structural biology, with a powerful specialize in functional concerns. The learn of membrane protein buildings not just presents a simple figuring out of existence on the molecular point but additionally is helping within the rational and precise layout of recent medicinal drugs with lowered unwanted side effects.

Extra info for Methods of Peptide Synthesis. The Peptides, Volume 1

Example text

Thiele and Dent (2307) used chloroform as solvent and carried out the reaction in a sealed tube at 60°-65°C. The compounds can be more readily obtained, ac­ cording to Carpenter and Gish (463), in dioxane at room temperature. p-Nitrocarbobenzoxy chloride is a stable crystalline compound. The blocked amino acids can be prepared by the standard procedures. The acid chloride is added in dioxane solution (463). The derivatives of serine, threonine, and tyrosine are best obtained in a dioxane-water mixture in the presence of equimolar amounts of alkali and sodium 32 I.

The same difficulties in purification and isolation are encountered. g. oxytocin or vasopressin). Birkhofer et al. (243) have described a decarbobenzoxylation by reduction with triethylsilane. It has been applied successfully to carbobenzoxy-S-benzylcysteine, with preservation of the S-benzyl protection. In addition to the protection of amino groups, phenolic hydroxyls (see Chapter IV,F,l,b,y) and the mercapto function (see Chapter IV, G,l,e) can also be blocked by the carbobenzoxy group. 2. Modified Carbobenzoxy Groups a.

This side reaction can be prevented by the addition of such compounds as phenol, resorcinol, or anisole that are very easily substituted. The reaction time varies from 12 to 150 minutes, depending on the amino acid used. Yields are 40-98%. g. O-benzyltyrosine) as well as benzyl esters. According to Khosla and Anand (1224b), decarbobenzoxylation occurs under even milder conditions (3 hours, 38°C). The benzyl, jo-nitrobenzyl, and methyl esters are cleaved in addition to the carbobenzoxy group, if a mixture of trifluoro­ acetic acid and hydrochloric acid is applied (4 hours, 40°C).

Download PDF sample

Rated 4.21 of 5 – based on 9 votes