Methods in Cilia & Flagella by Renata Basto, Wallace F. Marshall

By Renata Basto, Wallace F. Marshall

The objective of this booklet is to assemble equipment and protocols for learning cilia in a variety of diversified mobilephone kinds, in order that researchers from many fields of biology can commence exploring the position of cilia of their personal system.

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    Warn, R. , & Wehland, J. (1990). Distribution of microtubules containing post-translationally modified alpha-tubulin during Drosophila embryogenesis. Cell Motility and the Cytoskeleton, 17, 34e45. , & Boletta, A. (2009). A novel mouse model reveals that polycystin-1 deficiency in ependyma and choroid plexus results in dysfunctional cilia and hydrocephalus. PLoS One, 4, e7137. , et al. (1992). Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335.

    Journal of Cell Science, 108(Pt 9), 3013e3028. Magiera, M. , & Janke, C. (2013). Investigating tubulin posttranslational modifications with specific antibodies. Methods in Cell Biology, 115, 247e267. Marshall, W. , & Kintner, C. (2008). Cilia orientation and the fluid mechanics of development. Current Opinion in Cell Biology, 20, 48e52. , & Alvarez-Buylla, A. (2010). The subventricular zone en-face: wholemount staining and ependymal flow. Journal of Visualized Experiments. pii: 1938. 3791/1938.

    Wehland, J. (1990). Distribution of microtubules containing post-translationally modified alpha-tubulin during Drosophila embryogenesis. Cell Motility and the Cytoskeleton, 17, 34e45. , & Boletta, A. (2009). A novel mouse model reveals that polycystin-1 deficiency in ependyma and choroid plexus results in dysfunctional cilia and hydrocephalus. PLoS One, 4, e7137. , et al. (1992). Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335.

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