Lectins: Methods and Protocols by Jun Hirabayashi

By Jun Hirabayashi

Lectins: tools and Protocols summarizes vintage lectin applied sciences and complex innovations with excessive throughputs and sensitivities. Chapters comprise equipment and methods for serial lectin-affinity chromatography technique, lectin-probed western blot and histochemical analyses, quantitative interplay analyses according to equilibrium dialysis, isothermal calorimetry, floor plasmon resonance, innovations for elucidating capabilities of endogenous animal lectins, complicated equipment on “engineer” novel lectins via evolutionary techniques, and specified gear. Written within the hugely profitable Methods in Molecular Biology series layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and key pointers on troubleshooting and fending off recognized pitfalls.

Authoritative and useful, Lectins: tools and Protocols seeks to help not just lectin experts but additionally non-experts together with either younger scientists.

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Mol Vis 8:10–16 37 123. Houser J, Komarek J, Kostlanova N et al (2013) A soluble fucose-specific lectin from Aspergillus fumigatus conidia - structure, specificity and possible role in fungal pathogenicity. PLoS One 8:e83077 124. Rosen S, Bergstrom J, Karlsson KA et al (1996) A multispecific saline-soluble lectin from the parasitic fungus Arthrobotrys oligospora. Similarities in the binding specificities compared with a lectin from the mushroom agaricus bisporus. Eur J Biochem 238:830–837 125. Kossowska B, Lamer-Zarawska E, Olczak M et al (1999) Lectin from Beauveria bassiana mycelium recognizes Thomsen-Friedenreich antigen and related structures.

6 Marasmius oreades Agglutinin (MOA) The Scotch bonnet (Marasmius oreades) is known as a fairy ringforming mushroom. The lectin isolated from this organism (Marasmius oreades agglutinin, MOA) is a 30 kDa dimer composed of two subunits of approximately 15 kDa and is highly specific for Galα1-3Gal, Galα1-3Galβ1-4GlcNAc, and the blood group B determinant. MOA is an ideal reagent for detection and identification of Galα1-3Gal and Galα1-3Galβ1-4GlcNAc/Glc epitopes present on the chain ends of glycoproteins and glycolipids.

26. Agrocybe cylindracea. Int J Biol Macromol 42:138–144 Ngai PH, Zhao Z, Ng TB (2005) Agrocybin, an antifungal peptide from the edible mushroom Agrocybe cylindracea. Peptides 26: 191–196 Wang H, Ng TB, Liu Q (2002) Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom Agrocybe cylindracea. Life Sci 70:877–885 Yagi F, Hiroyama H, Kodama S (2001) Agrocybe cylindracea lectin is a member of the galectin family. Glycoconj J 18:745–749 Debray H, Montreuil J (1989) Aleuria aurantia agglutinin.

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