Interferons Part A by Sidney Pestka (Eds.)

By Sidney Pestka (Eds.)

The significantly acclaimed laboratory typical, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this day - actually an important booklet for researchers in all fields of existence sciences

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Extra resources for Interferons Part A

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Lmmunol. 118, 1931 (1977). 32 INDUCTION AND PRODUCTION OF INTERFERONS [4] WASTE F{G. 2. Continuous-flow centrifugation of leukocytes after treatment with NH4CI. the MSE rotor, the shaft hole is closed with a Teflon plug. The cells are then gently detached from the walls of the basket by the injection of cold phosphate-buffered saline (PBS) through a 21-gauge needle with a Cornwall syringe. 4, contains no calcium or magnesium and is supplemented with 25/~g of neomycin per milliliter. The cells in the basket are gently dispersed with a 20-ml pipette.

Cantell and D. R. Tovell, Appl. Microbiol. 22, 625 (1971). F. Wheelock, J. Bacteriol. 92, 1415 (1966). METHODS IN ENZYMOLOGY, VOL. 78 Copyright© 1981by AcademicPress, Inc. All rightsof reproductionin any form reserved. ISBN 0-12-181978-7 46 INDUCTION AND PRODUCTION OF INTERFERONS [6] Casein Suspension. ) in a 6-liter Erlenmeyer flask are dissolved in 3 liters of deionized distilled water and autoclaved for 12-15 min. The resultant solution is off white in color. As much as 30 liters of autoclaved milk are combined in a carboy after passage through several layers of cheesecloth to remove insoluble material generated by autoclaving.

A. A. Waldman, R. S. Miller, P. C. Familletti, S. Rubinstein, and S. Pestka, this volume [5]. 2 K. R. r H. s K. E. , McCredie, E. J. Freireich, J. P. Hester, and C. Vallejos, Transfusion 14, 357 (1974). H. Aster, H. E. Cooper, and D. L. Singer, J. Lab. Clin. Med. 63, 161 (1964). Strander and K. Cantell, Ann. Med. Exp. Biol. Fenn. 44, 265 (1966). Cantell and D. R. Tovell, Appl. Microbiol. 22, 625 (1971). F. Wheelock, J. Bacteriol. 92, 1415 (1966). METHODS IN ENZYMOLOGY, VOL. 78 Copyright© 1981by AcademicPress, Inc.

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