Inorganic chemical biology : principles, techniques and by Gilles Gasser

By Gilles Gasser

Knowing, making a choice on and influencing the organic platforms are the first goals of chemical biology. From this attitude, steel complexes have continually been of serious tips to chemical biologists, for instance, in structural identity and purification of crucial biomolecules, for visualizing mobile organelles or to inhibit particular enzymes. This inorganic part of chemical biology, which Read more...

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knowing, picking out and influencing the organic structures are the first pursuits of chemical biology. Read more...

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11, panel on the right). 11 Multi-dimensional immobilized metal ion affinity chromatography (MD-IMAC) for the separation of multiple bacterial secondary metabolites with differential metal ion affinities. In the MD-IMAC setup, two columns containing IMAC resins loaded with different metal ions are configured in series. After loading the mixture containing the analytes, the two columns are disassembled and the bound components are eluted from each column. The column order can affect the resolution: DFOB and bleomycin were well resolved using a Yb(III)-loaded resin mounted above a Cu(II)-loaded resin (left-hand side), but were not resolved using a Cu(II)-loaded resin mounted above a Yb(III)-loaded resin (right-hand side) example of the use of MD-IMAC and its underlying principle of separating non-protein based bacterial metabolites based upon differential metal ion affinities.

5)), which resulted in the liberation of biotin–DFOB as a metal-free adduct. The yield of biotin–DFOB was 76% and the product was significantly purer than the equivalent product prepared using traditional solution phase chemistry (Fig. 14). The veracity of the method depended upon the strength of the multiple coordinate bonds between DFOB and the immobilized Ni(II)–IDA chelate being functionally equivalent to a covalent bond, as used in traditional SPOS methods. Methods that use metabolites as molecular probes are keenly sought in metabolomics/proteomics applications [155–158], and CBD-SPOS delivers a new methodology to access these probes.

Compared with the free ligands, Fe(III)-loaded DFOB and DFOA1 eluted on the RP-HPLC column in a window described by increased water solubility, which is consistent with the role of siderophores to increase the water solubility of Fe(III). Adapted with permission from [55] © 2008 Royal Society of Chemistry from the ferrioxamine class of siderophores [19, 20, 56]. In the presence of added Fe(III), these peaks (C, boxed) shifted in a systematic fashion to a more hydrophilic region of the reverse-phase HPLC (RP-HPLC) trace, in accord with the role of siderophores to increase the water solubility of Fe(III).

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