By Vladimir V. Didenko (Editor)
In In Situ Detection of DNA harm: equipment and Protocols, Vladimir Didenko and a panel of specialists describe the entire significant in situ concepts for learning DNA harm and apoptosis, and the way techniques initially designed to label apoptotic cells can be utilized for DNA harm research (and vice versa). The effortlessly reproducible ideas provided right here comprise all these designed to observe particular unmarried- and double-stranded DNA breaks in tissue sections whereas utilizing polymerases, ligases, nucleases, and kinases. There also are novel instrumental concepts that use ultrasound for in vivo and in situ detection of apoptotic DNA harm, and in situ purposes of stream and laser-scanning cytometry for research of DNA strand breaks and apoptosis. state of the art and hugely useful, In Situ Detection of DNA harm: equipment and Protocols provides for the 1st time a whole set of enzymatic tools for picking out DNA breaks, and should drastically facilitate the paintings of all these learning the in situ detection of DNA harm and the labeling of apoptotic cells.
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Additional resources for In Situ Detection of DNA Damage: Methods and Protocols (Methods in Molecular Biology Vol 203)
Determining the Nature of the Ends of the Fragments 1. 15 mM spermine. 13 g spermidine (trihydrochloride, Sigma, St. 9 g NaCl. 4 and store at 4°C. 2. 25 mg/mL BSA. 54 mg DTT, 5 mg BSA. 6 and store at 4°C. 3. 2, 5 mM DTT, 10 mM MgCl2. 03 g MgCl2. 2. 4. 5% (w/v) Triton X100. 5 g Triton X 100. 5 and store at 4°C. 5. 5. 37 g EDTA. 5 with HCl and store at room temperature. 6. RNase solution: 2 mg/mL RNase A (Sigma, St. 5. 5 mg NaCl, 10 mg RNase A . 5 mL into microfuge tubes and store at –20°C. 7. 5% (w/v) N-Lauroylsarcosine.
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