Hydrophillic Interaction Liquid Chromatography (Hilic) and by Perry G. Wang, Weixuan He

By Perry G. Wang, Weixuan He

This is often the 1st publication that comprehensively and systematically describes the hot know-how of hydrophilic interplay liquid chromatography (HILIC). Hydrophilic interplay chromatography is a separation strategy appropriate for polar and hydrophilic compounds and orthogonal to reversed part liquid chromatography. From small natural molecules to proteins, the textual content explores the numerous purposes of HILIC within the analytical box. Winner of the President's Award for Excellence, the writer explains how HILIC can considerably increase analytical throughput via shortening pattern coaching technique, that is one of many bottlenecks for drug discovery and improvement within the pharmaceutical undefined.

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17 Retention maps for various structurally related peptides on a silica hydridebased C18 column. 1% formic acid. © 2011 by Taylor and Francis Group, LLC 24 Hydrophilic Interaction Liquid Chromatography and Advanced Applications hydrophobic species are well retained and can be separated. In the complementary analysis by ANP, the hydrophobic species would elute at or near the void volume while the hydrophilic species would be well retained and could be separated. Thus by two analyses of a protein digest, one RP and one ANP, the complete range of peptides could be determined.

These features are reviewed next in order to provide a better understanding of the ANP phenomena and as a guide for developing and optimizing ANP methods. 1 Column A number of silica hydride-based columns have been tested for ANP retention capabilities. 7 shows the retention for the amino sugar glucosamine on a silica hydride stationary phase with a bonded C18 moiety. This column is generally one that would be used for RP applications, and as expected it has substantial retention for a broad range of hydrophobic compounds as well.

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