Growth, Differentiation and Sexuality by S. A. MacNeill (auth.), Professor Dr. Joseph G. H. Wessels,

By S. A. MacNeill (auth.), Professor Dr. Joseph G. H. Wessels, Professor Dr. Friedhelm Meinhardt (eds.)

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IkJ} pypl ;~2 arrested in late G2 at a point prior to spindle formation and chromosome condensation (Fig. 1; Russell and Nurse 1986). Overproduction of the cdc25 protein leads to premature entry into mitosis and cell division at a reduced cell size (Table 3), indicating that the activity of this protein can be rate-limiting for mitotic entry (Russell and Nurse 1986), and purified cdc25 can dephosphorylate cdc2 on tyrosine 15 in vitro (Millar et al. 1991). The cdc25 protein function is dispensable when phosphorylation of cdc2 on tyrosine 15 is reduced (in cells lacking the weel tyrosine kinase, described below) or abolished (when tyrosine 15 is replaced with a non-phosphorylatable phenylalanine residue).

D. Retention Mechanisms of ER Resident Proteins. . . . . . . . . . . IV. Processing of Proteins in the Golgi Apparatus A. Elongation of N-Chains . . . . . . . . . . B. Elongation of O-Chains . . . . . . . . . . C. Proteolytic Processing ..................... V. Secretion of Cell Envelope Proteins. . . . . VI. Conclusions . . . . . . . . . . . . . . . References. . . . . . . . . . . . . . . . 25 25 26 26 27 28 28 28 29 29 29 30 30 30 31 32 32 34 34 34 36 36 36 37 37 I.

Academic Press, London, pp 431-452 Sorger PK, Murray A W (1992) S-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cDC28. Nature 355:365-371 Sudbery PE, Goodey AR, Carter BL (1980) Genes which control cell proliferation in the yeast Saccharomyces cerevisiae. Nature 288:401-404 Surana U, Robitsch H, Price C, Schuster T, Fitch I, Futcher AB, Nasmyth K (1991) The role of CDC28 and cyelins during mitosis in the budding yeast Saccharomyces cerevisiae. Cell 65:145-161 Surana U, Amon A, Dowzer C, McGrew J, Byers B, Nasmyth K (1993) Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.

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