Glutathione. Proceedings of the Symposium Held at by S. Colowick, D. R. Schwarz, A. Lazarow

By S. Colowick, D. R. Schwarz, A. Lazarow

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Under our experimental conditions, glutathione was in the form of a doubly charged anion. No effect of the anion of the guanidinium salt could therefore be expected. The ''denaturing'' effect of urea on glutathione led us to extend these experiments to other thiols with and without other functional groups. The results are listed in Table II. It can be seen from these results that there is a striking correlation between the potentiating effect of urea on the nitroprusside color and the complexity of the thiols.

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T. Edsall, in L. R. Ryklan and C. L. A. Schmidt, Arch. Biochem. 5, 89 (1944). 14. W. Stricks and I. M. Kolthoff, / . Am. Chem. Soc. 73, 4569 (1951); ibid. 75, 5673 (1953). 15. W. Gordy and S. C. Stanford, / . Am. Chem. Soc. 62, 497 (1940). 16. M. J. Copley, C. S. Marvel and E. Ginsberg, Am. Chem. Soc. 61, 3161 (1939). 17. G. Hopkins and L. Hunter, / . Chem. Soc. 638 (1942). 18. A. A. Burrows and L. Hunter, / . Chem. Soc. 4118 (1952). 19. H. Hoyer, Kolloid-Z. 122, 142 (1951). 20. R. Cecil, Biochem.

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