Flow Cytometry in Hematopathology (2nd Edition) by Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan

By Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan

The second one version of this quantity displays the new advances within the FCM research of hematopoietic problems. The chapters were revised to include new textual content and figures. the quantity is aimed toward hematopathologists, hematologists, pathologists, and laboratory technicians.

Show description

Read Online or Download Flow Cytometry in Hematopathology (2nd Edition) PDF

Best analysis books

Multidisciplinary Methods for Analysis Optimization and Control of Complex Systems

This publication involves lecture notes of a summer season tuition named after the overdue Jacques Louis Lions. The summer time institution used to be designed to alert either Academia and to the expanding function of multidisciplinary equipment and instruments for the layout of advanced items in a number of parts of socio-economic curiosity.

Additional info for Flow Cytometry in Hematopathology (2nd Edition)

Example text

Immunoglobulins have many epitopes and are, therefore, more easily detectable by polyclonal Fab′2 fragments than by monoclonal reagents. The surface immunoglobulins in some mature B-cell malignancies may not be produced correctly; one or more epitopes may be deleted or altered. Staining with a monoclonal antibody may yield a false-negative result if the reagent happens to be specific for the missing or modified epitope, whereas polyclonal antibodies, especially those with broader specificities, will give a positive result by reacting with the other epitopes of the immunoglobulin.

In the latter instance, a doublet is the result of two single particles being so close to each other in the sample core stream that the flow cytometer sees them as a single event. The diameter of the core stream and thereby the alignment of the cells is affected by the sample delivery rate into the flow cell; the higher the sample delivery rate, the wider the core stream and the lower the precision and accuracy of the data collected. Doublets and cell aggregates in the cell suspension can be the result of either delay in analysis or suboptimal tissue dissociation, in which case they are also present on the corresponding cytospins.

Based on this comparative evaluation of different brands of kappa and lambda, a given brand is considered optimal if the fluorescence signals fall in the expected range for any given disorder, namely weak intensity in CLL/SLL and moderate to strong in MCL, along with the least background staining. With some brands of kappa/lambda reagents, it may not be possible to achieve the appropriate fluorescence intensity. 4) and may fall in the same fluorescence range observed in FCC and MCL. The intensity of surface light chain expression is one of several criteria critical in the diagnosis and subclassification of mature B-cell malignancies.

Download PDF sample

Rated 4.28 of 5 – based on 16 votes