By Stefan Weber, Erik Schleicher
In Flavins and Flavoproteins: equipment and Protocols, specialist researchers within the box element the various tools that are now wide-spread to review flavins and flavoproteins. those contain evaluation type equipment and protocols to exemplify the diversity, the facility and the good fortune of recent concepts and strategies in program to flavoproteins. half I of this quantity covers common homes, syntheses and functions of unfastened flavins in addition to its analogs and flavoproteins. half II covers characterizations of flavins and flavoproteins utilizing glossy experimental concepts in addition to theoretical equipment. Written within the hugely winning Methods in Molecular Biology sequence structure, the chapters contain the type of certain description and implementation recommendation that's the most important for purchasing optimum ends up in the laboratory.
Thorough and intuitive, Flavins and Flavoproteins: equipment and Protocols aids scientists in carrying on with to take on the numerous questions that must be spoke back to extra totally understand the immense range and specificity of flavin-governed organic processes.
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Additional resources for Flavins and Flavoproteins: Methods and Protocols
Fischer M, Bacher A (2011) Biosynthesis of vitamin B2 and flavocoenzymes in plants. Adv Bot Res 58:93–152 10. Fischer M, Bacher A (2011) Biosynthesis of vitamin B2: a unique way to assemble a xylene ring. Chembiochem 12:670–680 11. Foor F, Brown GM (1975) Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli. J Biol Chem 250:3545–3551 12. Foor F, Brown GM (1980) GTP cyclohydrolase II from Escherichia coli. Methods Enzymol 66:303–307 13. Ritz H, Schramek N, Bracher A, Herz S, Eisenreich W, Richter G, Bacher A (2001) Biosynthesis of riboflavin: studies on the mechanism of GTP cyclohydrolase II.
Subtilis, was functionally characterized, and was found to encode a transporter for RF and RoF . Importantly, RF transporters have also been described in eukaryotes. In Saccharomyces cerevisiae the RF transporter Mch5p was identified .
Photosynth Res 20:843–850 Braden BC, Velikovsky CA, Cauerhff AA, Polikarpov I, Goldbaum FA (2000) Divergence in macromolecular assembly: X-ray crystallographic structure analysis of lumazine synthase from Brucella abortus. J Mol Biol 297: 1031–1036 Gerhardt S, Haase I, Steinbacher S, Kaiser JT, Cushman M, Bacher A, Huber R, Fischer M (2002) The structural basis of riboflavin binding to Schizosaccharomyces pombe 6,7-dimethyl8-ribityllumazine synthase. J Mol Biol 318: 1317–1329 Klinke S, Zylberman V, Bonomi HR, Haase I, Guimaraes BG, Braden BC, Bacher A, Fischer M, Goldbaum FA (2007) Structural and kinetic properties of lumazine synthase isoenzymes in the order Rhizobiales.