Expression of Recombinant Genes in Eukaryotic Systems by Glorioso J.C., Schmidt M.C., Abelson J.N. (eds.)

By Glorioso J.C., Schmidt M.C., Abelson J.N. (eds.)

The severely acclaimed laboratory typical for greater than 40 years, equipment in Enzymology is among the so much hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless correct this present day - really an important ebook for researchers in all fields of lifestyles sciences.Key positive factors* research of Gene Expression* Gene Expression platforms for useful research* Yeast Expression platforms* RNA-based keep an eye on of Recombinant Gene Expression* Small Molecule keep an eye on of Gene Expression* Viral platforms for Recombinant Gene Expression

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Homologous recombination is less facile than other systems, but has the major advantage of providing definitive information about the functional role of an element in its normal chromosomal context. To fully appreciate the advantages of using homologous recombination (HR), it is necessary to understand the limitations of the other available approaches. The ability to analyze specific interactions between purified proteins and nucleic acids makes biochemical analysis powerful, but these interactions often do not faithfully mimic the complexities seen in intact cells.

50 S. Dymecki, Proc. Natl. Acad. A. 93, 6191 (1996). 5~M. Lakso, J. Pichel, J. Gorman, B. Sauer, Y. Okamoto, E. Lee, F. Alt, and H. Westphal, Proc. Natl. Acad. Sci. A. 93, 5860 (1996). 52 p. Orban, D. Chui, and J. Marth, Proc. Natl. Acad. Sci. A. 89, 6861 (1992). s3 F. Schwenk, U. Baron, and K. Rajewsky, Nucleic Acids Res. 23, 5080 (1995). [3] A 51 MAMMALIAN CIS-REGULATORY D N A ELEMENTS genomic int~ration ,,te # l expressi recombinase on -I- genomi c integration B site T expression recombinase /t • 4- // FIG.

Bouhassira, K. Westerman, and P. Leboulch, Blood 90, 3332 (1997). 59 B. Sauer, J. Mol. Biol. 223, 911 (1992). 6o A. Nagy, C. Moens, E. Ivanyi, P. J, M. Gertsenstein, A. Hadjantonakis, M. Pirity, and J. Rossant, Curr. Biol. 8, 661 (1998). 6l K. Araki, M. Araki, J. Miyazaki, and P, Vassalli, Proc. Natl. Acad. Sci. A. 92, 160 (1995). 54 ANALYSIS OF GENE EXPRESSION [3] by breeding with a Cre recombinase-expressing transgenic line. Mice carrying both transgenes are identified by Southern blot or PCR analysis of tail DNA.

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