By M. De Brabander, R. Nuydens, H. Geerts (auth.), Pierre J. Courtoy (eds.)
(Director: Pierre J. COURTOY) years after its first collecting in Oeiras, Portugal, the ecu Endocytosis staff convened for a moment workshop on the Pasteur Institute, Paris, on October 1-5, 1990. The assembly is suggested intimately during this quantity; a initial assurance, in line with the overviews of every consultation, has seemed within the New Biologist (1991, 3:243-252). the 3 major ambitions, to develop the viewers, to provide a extra accomplished view of the a number of features of endocytosis, from simple biology to wellbeing and fitness, illness and treatment, in addition to to elucidate debatable matters, were mostly fulfilled. the second one eu Workshop on Endocytosis used to be attended through greater than tOO individuals, originating from 18 nations. fifty nine lectures and 35 posters have been awarded. moreover, vivi~ roundtables allowed to entirely talk about the dynamics and the legislation of the endocytic gear, in addition to the function of endocytosis in antigen presentation. Endocytosis is a common and certain estate of all eukaryotic cells, together with protists, crops and fungi.
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Extra resources for Endocytosis: From Cell Biology to Health, Disease and Therapy
B. Correlation between the number of CR1 per cell and the number of gold particles per 10llm2. , 1990) These morphological observations were correlated with measurements of C3bdependent immune complexes binding. To that end, fMLP-stimulated PMN were challenged at 4°C with purified opsonized immune complexes which bound 20 essentially via CR1 (Paccaud J-P et ai, 1990). At equal number of receptors, immune complexes adhered less well to neutrophils than to erythrocytes. This functional difference could be attributed to the low level of CR1 clustering in neutrophils as compared to the one observed in erythrocytes.
Stossel TP (1988) The mechanical responses of white blood cells in: Inflammation basic principles and clinical correlates (eds) Gallin JI, Golstein 1M, Snyderman R. Raven Press New York. Unkeless JC, Wright SO (1988) Phagocytic cells and complement receptors in: Inflammation basic principles and clinical correlates (eds) Gallin JI, Golstein 1M, Snyderman R. Raven Press New York. Wong WW, Cahill JM, Rosen MD, Kennedy CA, Bonaccio ET, Morris MJ, Wilson JG, Klickstein LB, Fearon DT (1989) Structure of the human CR1 gene.
Interestingly the amount of free clathrin was differentially affected. Whereas at low pH or in ATP depleted cells the quantity of free clathrin was unchanged, it was reduced at 4°C concomitantly to an increase in the number of coated pits at the level of the plasma membrane. Free clathrin was also absent when cells were incubated in hypertonic medium. These results are in agreement with the morphological observations reported by the group of J. Heuser (Heuser and Kirchausen, 1985; Heuser and Keen, 1988; Heuser, 1989; Heuser and Anderson, 1989).