By Yunhan Hong, Manfred Schartl (auth.), Kursad Turksen (eds.)
Drawing at the dramatically expanding study on embryonic stem (ES) telephone biology and differentiation, Kursad Turksen has thoroughly up-to-date and accelerated his hugely acclaimed first variation of Embryonic Stem Cells: equipment and Protocols into volumes. concentrating on ES cells lately remoted from varied nonhuman species, quantity considered one of Embryonic Stem mobilephone Protocols: Isolation and Characterization, moment version, offers a various number of simply reproducible mobile and molecular protocols for the isolation, upkeep, and characterization of embryonic stem cells. A significant other moment quantity, Embryonic Stem telephone Protocols: Differentiation versions, moment variation, covers state of the art equipment for deriving many varieties of differentiating cells from ES cells. The protocols stick with the profitable equipment in Molecular Biology™ sequence layout, each one providing step by step laboratory directions, an creation outlining the foundations at the back of the method, lists of the mandatory apparatus and reagents, and tips about troubleshooting and heading off identified pitfalls.
Authoritative and state of the art, the 2 volumes of Embryonic Stem Cells remove darkness from for either beginners and specialists not just our present knowing of the biology of embryonic stem cells and their application in common tissue homeostasis and regenerative drugs functions, but additionally supply special bills of the instruments required for profitable paintings within the area.
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Additional resources for Embryonic Stem Cell Protocols: Volume 1: Isolation and Characterization
No. U3003). 14. 100 µM nucleotide stock solution. For 100 mL: mix 80 mg adenosine, 73 mg cytidine, 85 mg guanosine, 24 mg thymidine, and 73 mg uridine in 100 mL dH2O, and dissolve the nucleotide mixture at 37°C. 22-µm disposable filter, dispense the solution into 1-mL aliquots, and store at Ϫ20°C. Thaw nucleotide stock at 37°C before use. 15. Fertilized, freshly laid chicken eggs. 16. Lipopolysaccharide (LPS) from E. coli serotype O127:B8 (100 mg; Sigma, cat. no. L3880). 17. Penicillin-streptomycin 100X (100 mL; Gibco BRL, cat.
Heart induction: embryology to cardiomyocyte regener12. ation. Trends Caridiovasc. Med. 14, 121–125. , and Schartl, M. (2003) Mitf expression is sufficient to direct differentia13. tion of medaka blastula derived stem cells to melanocytes. Development 130, 6545–6553. , et al. (1993) Generation of germline chimeras in 14. medaka (Oryzias latipes). Mol. Mar. Biol. Biotechnol. 2, 325–332. , and Schartl, M. (1998) Efficiency of cell culture derivation from blas15. tula embryos and of chimera formation in the medaka (Oryzias latipes) depends on donor genotype and passage number.
Biotechnol. 2, 325–332. , and Schartl, M. (1998) Efficiency of cell culture derivation from blas15. tula embryos and of chimera formation in the medaka (Oryzias latipes) depends on donor genotype and passage number. Dev. Genes Evol. 208, 595–602. , et al. (2001) Fertile and diploid nuclear transplants 16. derived from embryonic cells of a small laboratory fish, medaka (Oryzias latipes). Proc. Natl. Acad. Sci. USA 98, 1071–1076. , et al. (2004) Establishment of a normal medakafish spermatogo17. nial cell line capable of sperm production in vitro.