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Hall, R. , M. J. Robins, L. Stasiuk and R. Thedford. 1966. Isolation of N 6 -Cr, r-dimethylallyl) adenosine from soluble ribonucleicacid. J. Amer Ghem. , 88: 2614-2615. Hardin. J. O'Brien and J. H. Cherry. 1970. Stimulation of chromatin-bound RNA polymerase activity by a soluble factor. Biochim. Biophys. Acta, 224: 667-670. Higgins, T. J. , J. A. Zwar and J. V. Jacobsen. 1976. Gibberellic acid enhances the level of translatable mRNA for IX-amylase in barely aleurone layers. Nature (London) 260: 166-169.
Determine the functions of polypeptides encoded by the hormoneregulated mRNAs. An initial approach might be to use prokaryotic expression vectors to produce quantities of the polypeptides sufficient to raise antibodies, the antibodies then being used to localize the hormoneregulated polypeptide in responsive cells. 4. Analyze hormone promoters utilizing hybrid construction. For example, putative hormonal promoters might be inserted into the T-DNA of Agrobacterium tumefaciens, with subsequent transformation of plants.
Mickiewicz Univ. Poznan, Ser. , 16. pp. 1-63. Gwozdz, E. A. and A. M. Szweykowska. 1982. Cytokinin-induced activation of polyribosomes in the protonema of Ceratoe/on purpureus. Physiol. , 55: 214-220. Gwozdz, E. A. and A. Wozny. 1983. Cytokinin-controlled polyribosome formation and protein synthesis in cucumber cotyledons. Physiol. , 59: 103-110. , H. Menzel and A. Krauss. 1971. Veruche und Hypothese zur Primärwirkung des Auxins beim Streckungswachstum. Planta, 100: 47-75. 32 Hall, R. H. 1973. Cytokinins as a probe of developmental processes.