By R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach
This book collects the best and state-of-the-art equipment and protocols for deriving and culturing human embryonic and grownup stem cells—in one convenient resource.
This groundbreaking book follows the culture of earlier books within the tradition of specialised Cells Series—each tools and protocols bankruptcy is laid out precisely just like the subsequent, with stepwise protocols, preceded via particular standards for that protocol, and a concise dialogue of tools illustrated via info. The editors describe a constrained variety of consultant recommendations throughout a large spectrum of stem cells from embryonic, child, and grownup tissue, yielding an all-encompassing and flexible advisor to the sector of stem cellphone biology and culture.
The booklet incorporates a accomplished checklist of providers for all gear utilized in the protocols awarded, with web pages on hand in an appendix. also, there's a bankruptcy on qc, and different chapters overlaying felony and moral matters, cryopreservation, and feeder layer culture. This textual content is a one-stop source for all researchers, scientific scientists, academics, and scholars inquisitive about this significant zone of research.
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Additional info for Culture of Human Stem Cells (Culture of Specialized Cells)
And Auerbach, J. M. (2006). A molecular scheme for improved characterization of human embryonic stem cell lines. BMC Biol 4:28. , and Hakomori S. (1982). A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to developmentally regulated antigen SSEA-1. J Biol. Chem. 257:14865–14874. , Solter D. (1983). Stage-speciﬁc embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 2:2355–2361.
Cut through the skin and tissue to expose the uterus. Remove the uterine horns and place in a 10-cm plastic Petri dish containing PBSA. Remove the embryos from the embryonic sac with a sterile scalpel and discard all other tissue including the placenta and membranes. Remove the top of the head containing the brain and discard. Dissect the embryos by making a slit along the central axis from head to tail along the front of each pup, exposing and discarding the internal organs. Place the remainder of the embryos in a 50-mL centrifuge tube and wash 4 times with PBSA.
2005]. This highlights the need to assess routinely the karyotype of each hES cell line to ensure that the cells have not adapted to culture by undergoing chromosomal alterations. , new medium formulation, new sources, or changes in feeder species). 3. Differentiation of Human Embryonic Stem Cells Pluripotent ES cells are capable of generating a wide variety of somatic cell types both in vitro and in vivo. In vitro, pluripotency can be demonstrated by assessing the phenotype of cells within colonies that have differentiated spontaneously, or by growing the cells to conﬂuence, thus promoting forced differentiation.