Ciba Foundation Symposium - Isotopes in Biochemistry

Content:
Chapter 1 Chairman's starting feedback (pages 1–3): A. S. McFarlane
Chapter 2 Metabolism of 14C?Labelled Steroids (pages 4–13): C. P. Leblond
Chapter three excessive ldl cholesterol content material of Human Spleen (pages 14–16): Karl Bernhard
Chapter four The Biosynthesis of Radioactive ldl cholesterol through Surviving Liver Slices (pages 17–27): Samuel Gurin and Roscoe O. Brady
Chapter five reviews with Deuterium Steroids (pages 28–40): T. F. Gallagher
Chapter 6 The Biosynthetic Mechanism of Porphyrin Formation (pages 41–67): David Shemin and Jonathan Wittenberg
Chapter 7 reports on Mammalian crimson Cells (pages 68–85): A. Neubergeb
Chapter eight initial Investigations for a learn of strength used by the Surviving chook Erythrocyte in H?m Synthesis (pages 86–89): C. Rimington
Chapter nine Iron Metabolism in Pathological stipulations (pages 90–95): A. Vannotti
Chapter 10 The amendment of X?Ray Sensitivity by way of chemical substances (pages 96–113): Alexander Hollaender, G. E. Stapleton and W. T. Burnett
Chapter eleven influence of X?Rays on Nucleic Acid and Protein Synthesis within the Jensen Rat Sarcoma (pages 114–121): Barbara E. Holmes
Chapter 12 Radiation Dose in Tracer Experiments concerning Autoradiography (pages 122–137): S. R. Pelc
Chapter thirteen Synthesis of Deoxyribose Nucleic Acid and Nuclear Incorporation of 35S as proven by means of Autoradiographs (pages 138–151): Alma Howard and S. R. Pelc
Chapter 14 The Biosynthesis of Pyrimidines in vitro (pages 152–163): D. Wright Wilson
Chapter 15 reviews with natural? and Bio?Synthetic Nucleosides and Nucleotides (pages 164–174): G. B. Brown
Chapter sixteen using Radiophosphorus within the research of the Nucleic Acids (pages 175–183): J. N. Davidson
Chapter 17 price of Synthesis and Quantitative adaptations of the Ribonucleic Acid in the course of the development of a tradition of Polytomella Coeca (pages 184–189): R. Jeener
Chapter 18 a mode for the evaluate of the speed of Protein Synthesis in guy (pages 190–202): D. Rittenberg
Chapter 19 Turnover premiums in the course of Formation of Proteins and Polynucleotides in Regenerating Tissues (pages 203–212): E. Hammarsten
Chapter 20 Synthesis of Phenylalanine and Tyrosine in Yeast (pages 213–226): Konrad Bloch
Chapter 21 A research of Acetone Metabolism utilizing Glycogen and Serine as symptoms, and the function of C1?Compounds in Metabolism (pages 227–245): Harland G. Wood
Chapter 22 uneven Citric Acid (pages 246–257): Charles Heidelberger and Van R. Potter
Chapter 23 Mode of Formation of Fatty Acids from Acetate and Glucose as Studied within the Mammary Gland (pages 258–284): G. Popjak

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Preparation of radioactive 3 ,-hydroxypregnane-l1:20dione by the platinum catalysfid exchange reaction. I FHr GAc $*3 c=o C-3 HC 3 90X109 DISlNT/rnin/mm CORTISONE ACETATE rn p 245-246‘ ELls~16,100 [LID= +186°(ACETONE) 2 82x109 oIS,NT/m,”,rnm [ L I D . 5. Preparation of radioactive cortisone acetate. reaction, while useful for the biological studies, was not as high as we had anticipated from the results of Bloch and Rittenberg (1943)with cholesterol. These authors had found that cholesterol stably labelled with deuterium by the a STEROIDS 33 exchange reaction had an approximately equal distribution of the isotope in the nucleus and in the side chain.

F. GALLAGHER to the hormones we wished to prepare. The platinumcatalysed exchange reaction first studied in sterols by Bloch and Rittenberg seemed to offer the means for this end. It was found that, with suitable compounds, the incorporation of isotope was satisfactory and the yield was good. The best yields were obtained with unsaturated ketones of the type exemplified by androst-4-ene-3:17-dione. This compound upon heating in 70 per cent deuterated acetic acid and CH3 dP - 11,12 cn. 12 ETIOCHOLANOLONE FIG.

Of aetiocholanolone had been combined. Adding these values to the weight of pure androsterone removed from the first chromatogram ( 9 . ) afforded the total excretion of these two compounds. Since the total amount and the dilution were known, both endogenous production and the quantity obtained from the administered hormone were calculated. These results are shown in Table I. 5 mg. 8 mg. 8 = 0 * 6 mg. I ~ Wt. in mg. Btiocholanolone . . . . “Endogenous” . . . . Androsterone ........ “Endogenous” .

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