Cells and Tissues in Culture Methods, Biology and Physiology by E. N. Willmer

By E. N. Willmer

Cells and Tissues in tradition: tools, Biology, and body structure, quantity three specializes in the purposes of the tools of tissue tradition to numerous fields of research, together with virology, immunology, and preventive drugs.
The choice first deals info on molecular association of cells and tissues in tradition and tissue tradition in radiobiology. subject matters contain mobile association on the molecular point, fibrogenesis in tissue tradition, influence of radiation at the development of remoted cells, and irradiation of the chosen components of the phone. The ebook then considers the consequences of invading organisms on cells and tissues in tradition and phone, tissue, and organ cultures in virus learn.
The e-book elaborates on antibody construction in tissue tradition and tissue tradition in pharmacology. Discussions specialize in early makes an attempt at in vitro stories, tissue tradition within the examine of pharmacologically lively brokers, and strategies of evaluation of drug task. The textual content additionally reports invertebrate tissue and organ tradition in mobilephone study; creation and strategies hired in plant tissue tradition; and progress, differentiation and organogenesis in plant tissue and organ cultures.
The choice is a crucial resource of information for readers drawn to the tradition of cells and tissues.

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Extra resources for Cells and Tissues in Culture Methods, Biology and Physiology

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Part of the nuclear membrane (m) can be seen on the right, and the dense mass represents part of a nucleolus (n). x 105,000. has suggested that a diaphragm is present; either structural arrangement could help in the regulation of material passing through the pore. D . T H E F I N E - S T R U C T U R E OF TISSUE C U L T U R E CELLS D U R I N G MITOSIS Many studies have been made of living tissue culture cells in mitosis, with particular reference to the movement of chromosomes and the spindle (see Vol.

H. E. Huxley and Quart. J. micr. Sei. During anaphase, Barnicott and Huxley (1965) have observed that in newt cells the chromosomes first become more compact, and that, although the fibre bundles connecting the centromeres and centrioles must shorten by about one-third to one-half of their length at metaphase, their diameter was not increased. There was no evidence to suggest that the anaphase chromosomes were composed of two chromatids. The subsequent separation of the daughter cells was accompanied by an elongation of the spindle in the intervening regions.

The cells were obtained by trypsinization of 11-day-old chick embryos and the 38 SYLVIA FITTON JACKSON FIG. 17. Electron-micrograph of part of an osteoblastic culture grown for 48 h. Many collagen fibrils are visible between the cells, x 5,500. resultant cultures were found to incorporate 14 C-proline and synthesize 14 C-hydroxyproline into non-dialysable compounds. Further investigations of primary cultures of cells derived from frontal bone and from epiphyseal cartilage of 10 - 11-day-old chick embryos have been made to see whether the mode of formation of collagen fibrils in an osteogenic system differed from that in a chondrogenic system (Fitton Jackson, 1965).

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