Biomembranes Part L: Membrane Biogenesis: Processing and by Nathan P. Kaplan, Nathan P. Colowick, Sidney Fleischer,

By Nathan P. Kaplan, Nathan P. Colowick, Sidney Fleischer, Becca Fleischer

FROM THE PREFACE: Volumes ninety six to ninety eight, components J, ok, and L of the Biomembranes sequence, concentrate on method to review membrane biogenesis, meeting, concentrating on, and recycling. This box is without doubt one of the very fascinating and lively parts of study. destiny volumes will care for shipping and different facets of membrane functionality

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Achord, and W. S. Sly, Proc. Natl. Acad. Sci. A. 74, 2026 (1977). 9 I. Tabas and S. Kornfeld, J. Biol. Chem. 255, 6633 (1980). ~oB. Gumbiner and R. Kelly, Proc. Natl. Acad. Sci. A. 78, 318 (1981). ~1 M. Schmidt and M. Schlesinger, J. Biol. Chem. 255, 3334 (1980). ~2j. Lenard, Annu. Rev. Biophys. Bioeng. 7, 139 (1978). ~3p. Novick, C. Field, and R. Schekman, Cell 21,205 (1980). 14L. A. Hunt and D. F. Summers, J. Virol. 20, 646 (1976). 15 F. N. Katz and H. F. Lodish, J. CellBiol. 80, 416 (1979). 16 D.

_L/I ! ~ PERSPEX CELL SUSPENSION FIG. 3. (a) The experimental setup used to expose a suspension of cells to a brief electric field. (b) The time course of the electric field through the cell suspension made up in a glutamate solution as described in Table I when the applied high voltage is 2 kV and t h e capacitor 2 gF. The metal electrodes were stainless steel. The following points must also be borne in mind (for further details, see Knight4). 1. The duration o f exposure to the field must be long enough for the m e m b r a n e to charge up.

73, 156 (1976). 28 SPECIALIZED METHODS [3] protein discharge; (b) defined the role of intracellular and extracellular calcium during stimulus-secretion coupling10; (c) identified changes in cellular cyclic nucleotide levels during cholinergic and hormonal stimulation~2; and (d) outlined mechanisms for the appearance of both parallel and nonparallel discharge of secretory proteins in the exocrine pancreas. 8,13 ~3 G. A. Scheele, Am. J. Physiol. 238, 6467 (1980). [3] H i g h - V o l t a g e T e c h n i q u e s f o r G a i n i n g A c c e s s t o t h e Interior of Cells: Application to the Study of Exocytosis and Membrane Turnover By P.

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