Biochemistry Labfax by Ali D. Hames, J. A.A. Chambers, D. Rickwood

By Ali D. Hames, J. A.A. Chambers, D. Rickwood

LABFAX volumes are purpose-designed information reference books for training scientists. each one e-book provides key info for an immense topic in a single position and so saves hours of looking. It doesn't easily acquire jointly information that are already on hand in catalogues considering that those are frequently incomplete and will comprise conflicting info. relatively, the authors and editors of every LABFAX quantity have searched the unique literature for the actual facts which they understand the professional needs.
Biochemistry LabFax is a close compendium of crucial details - on radioisotopes, enzymes, proteins, nucleic acids, lipids, plus information on chosen concepts comparable to chromatography, electrophoresis, and so on. - wanted on a virtually day-by-day foundation via researchers in any quarter of biochemistry. additionally, the ebook acts as a gateway to different assets for info extra particular than could be coated in a quantity of this dimension

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RADIOISOTOPES USED IN BIOCHEMISTRY The characteristics of the isotopes used most frequently in biochemistry are listed in Table 1. RADIOISOTOPES 37 Table 1. 25(14%) LS,SS LS, C, SS Abbreviations: LS, liquid scintillation counting; SS, gamma counter (solid scintillant); C, Cerenkov; K, electron capture. 38 BIOCHEMISTRY LABFAX 3. RADIOACTIVE DECAY CORRECTION Some radioisotopes decay so quickly that it is necessary to apply corrections to the original activities. Table 2 gives an indication of the decay of various isotopes.

5 4-6 4-6 4-6 Approx. bed volume (mlg "') Table 1. 5-30 Peptides, globular proteins — — — — Dextrans Fractionation range (x 103) 50-150 100-300 50-150 100-300 (jtm) Dry bead diameter Sephacryl Cross-linked copolymer of allyl dextran and N,N' -methylenebis(acrylamide) S-100 high resolution 1-100 — 25-75 S-200 superfine 5-250 1-80 40-105 S-200 high resolution 5-250 1-80 25-75 S-300 superfine 10-1500 1-400 40-105 S-300 high resolution 10-1500 1-400 25-75 S-400 superfine 20-8000 10-2000 40-105 S-400 high resolution 20-8000 10-2000 25-75 S-500 superfine 40-20 xlO 3 — 40-105 S-500 high resolution 40-20 x l 03 — 25-75 S-1000 superfine 5000-10 4 — 40-105 PDX Cross-linked dextran beads GF-25 GF-25 GF-50 GF-50 Names and types of matrix Table 1.

4. Gray This is an absorbed dose of 1 joule kg~' of tissue. 1 gray equals 100 rads. 5. Dose equivalent man (sievert) This is defined as: (dose in Sv) = (dose in gray) x (quality factor, Q). The value of Q is unity for B particles, X-rays and y-rays, and 10 for a-particles, neutrons and protons. 100 rem is equal to 1 sievert (Sv). 2. RADIOISOTOPES USED IN BIOCHEMISTRY The characteristics of the isotopes used most frequently in biochemistry are listed in Table 1. RADIOISOTOPES 37 Table 1. 25(14%) LS,SS LS, C, SS Abbreviations: LS, liquid scintillation counting; SS, gamma counter (solid scintillant); C, Cerenkov; K, electron capture.

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