Bioanalytical applications of enzymes by Clarence H. Suelter, L. J. Kricka

By Clarence H. Suelter, L. J. Kricka

Information the newest advances in bioanalytical functions utilizing enzymes--techniques which are turning into more and more vital in research, synthesis, production and clinical prognosis. includes seven articles which disguise: enzyme categorized antibodies in bioassays, DNA restrict enzymes and RFLPs in medication, enzyme-labeled probes for nucleic acid hybridization, special methodologies of immobilized proteins in bioanalytical platforms, dry reagent chemistry basics, the speculation and functions of enzyme electrode biosenors, and advances in enzymatically coupled box influence transistors.

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This was achieved by coupling PEG to the molecule. To determine the partition constant accurately, 1251-labeled,PEGmodified lectin is used. The parti- M. GUPTA AND 0. 031 for native Con A. This means that the modification changed the partion constant by almost 2600 times. A fixed amount of the glycoprotein (HRPJand varying amounts of a free carbohydrate with specificity for Con A are mixed. The modified Con A is added. After the binding reaction is complete, partitioning is carried out in the twophase system.

3. ) TABLE 4 Partition Affinity Ligand Assay: Imrnunoassays of Different Antigens Type of Antigen Hydrophobic hapten Antigen Digoxin T3 Protein Cells T4 &-Microglobulin Staphylococci Streatococci Source: Adapted from Ling and Mattiasson (102). 5 x 103-104 UNIQUE APPLICATIONS OF IMMOBILIZED PROTEINS IN BIOANALYTICAL SYSTEMS 17. 19 PROTEIN IMMOBILIZATION ON MAGNETIC SUPPORTS An immobilized enzyme reagent can be recovered and reused. This separation normally involves centrifugation o r filtration.

Tramper, Biotechnol. Bioeng. 27, 1258-1269 (1985). 119. Reslow, P. Adlercreutz, and B. Mattiasson, ApP1. Microbiol. Biotechnol. 26, 1-8 (1987). 120. M. Reslow, P. Adlercreutz, and B. J. Biochem. 172,573-578 (1988). 121. M. Reslow, P. Adlercreutz, and B. Mattiasson, Eur. J. Biochem. 177, 313-318 (1988). 122. R. Kazandjian, J. S. Dordick, and A. M. Klibanov, Biotechnol. Bioeng. 28,417-421 (1986). 123. F. Hall, D. J . Best, and A. P. F. Turner, Anal. Chim. Acta 213, 113-119 (1988). 124. A. Miyabayashi, M.

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