By George A. Boyd
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Extra resources for Autoradiography in Biology and Medicine
The removal requires time. The more rapidly a fresh water layer can be presented to the emulsion surface, the more efficient will be the washing in a certain time, or the shorter will be the washing to a desired efficiency. The rapidity of changing the water layer is of course related to the rate of flow through the washing vessel. However, if several slides are stacked so closely that the resistance to flow is great and there is prac tically no movement of water between adjacent slides, a rapid flow THE PHOTOGRAPHIC PROCESS 39 through the vessel is of little value.
However, microscopic autoradiograms should be washed in cleaner water than is found in many cities. Distilled, filtered, or water passed through a small ion exchange column is preferable. Unclean water deposits algae, river or lake sedi ment, pipe rust, or other microscopic particulate matter upon the emulsion. These interfere with the interpretation of microscopic autoradiograms since some cannot be easily distinguished from a developed silver grain. I cannot overstress the necessity for clean wash water for good autoradiographic preparations.
The characteristic curves for the Type K X-ray film and Type NTB stripping film were not given, only the sensitivities. The data in Tables 1 and 2 were calculated from the above to show examples of the relative responses of some films to beta particles and/or photons of different energies from various isotopes. Furthermore, they will show that the sequence of response as a function of the maximum energy is the same for the four films. Two of these were double-coated 7 8 9 L0G, 0 DISINTEGRATIONS PER CM 2 10 n FIG.