Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, delivering researchers entry to authoritative reports of the newest discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unmatched view of the old improvement of enzymology. The sequence deals researchers the most recent knowing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic method, and their program in either the laboratory and undefined. every one quantity within the sequence positive aspects contributions by way of prime pioneers and investigators within the box from worldwide. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide selection of subject matters and lengthy old pedigree, Advances in Enzymology and similar parts of Molecular Biology can be utilized not just by way of scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition via any scientist drawn to the invention of an enzyme, its homes, and its applications.

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Extra resources for Advances in Enzymology and Related Areas of Molecular Biology, Volume 48

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FLOWERS A N D NATHAN SHARON zyme preparations was obtained by using as inhibitors low-molecularweight products of the hydrolytic reaction, particularly free monosaccharides. The activity of an impure enzyme, containing more than one glycosidase activity, may be controlled by the addition of such inhibitors to the substrate to prevent further degradation of the product of the desired enzymatic reaction. This approach was employed successfully in early work on the structure of secreted bloodgroup glycoproteins (see below).

Unpublished data. 102. Stadtman, T. , unpublished data. 103. Huber, R. , and Criddle, R. ,Arch. Biochem. B i q h y . , 122, 164-173 (1967). 104. Giinther, W. H. J. Org. , 32, 3931-3933 (1967). Advances in Enzymology and Related Areas ofbfolecular Biology, Volume46 Edited by F. F. Nord Copyright © 1979 by John Wiley & Sons, Inc. GLYCOSIDASES-PROPERTIES A N D APPLICATION T O T H E STUDY O F COMPLEX CARBOHYDRATES A N D CELL SURFACES By HAROLD M. FLOWERS AND NATHAN SHARON, Rehovot. Israel C0N TEN TS I.

The activity of an impure enzyme, containing more than one glycosidase activity, may be controlled by the addition of such inhibitors to the substrate to prevent further degradation of the product of the desired enzymatic reaction. This approach was employed successfully in early work on the structure of secreted bloodgroup glycoproteins (see below). B. COMPLEX SUBSTRATES Great advances have been made in recent years in the application of glycosidases to the analysis of the structure of complex carbohydrates (132).

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