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Additional resources for Advanced Bacterial Genetics
EMBO J. 11, 741–750. , and Lambowitz, A. (2002). ’’ ASM Press, Washington, DC. , and Botstein, D (1977). Genetic engineering in vivo using translocatable drug‐resistance elements. New methods in bacterial genetics. J. Mol. Biol. 116, 125–159. , and Gottesman, S. (1991). Uses of transposons with emphasis on Tn10. Methods Enzymol. 204, 139–180.  In Vivo Mutagenesis Using EZ‐Tn5TM By JOHN R. KIRBY Abstract Epicentre Biotechnologies has developed a suite of transposon‐based tools for use in modern bacterial genetics.
4] Identification of Essential Genes in Bacteria By DAVID R. HILLYARD and MICHAEL J. REDD Abstract Essential genes are identified in duplicated regions of the bacterial chromosome. Transposition of a vector that forms operon fusions into a strain carrying a chromosomal duplication allows insertion of the transposon into essential genes because a second copy of the essential gene is present. When the duplication is allowed to segregate, only the segregant that carries the copy of the intact essential gene survives.
3] IN VIVO mutagenesis using EZ‐Tn5tm 17 adjacent to its transposon target under conditions where the transposase is repressed. Introduction of the DNA by transduction, transformation, or electroporation into recipient cells allows for the induction of transposase that can then act on the adjacent transposon for transposition in the recipient cell. At some point, the remaining DNA including the transposase gene is degraded by nucleases resulting in a transposon insertion that is incapable of further transposition.